Detection of mouse natural killer T follicular helper (NKT(FH)) cells by flow cytometry.

ABSTRACT

Natural killer T follicular helper (NKT(FH)) cell, a recently identified B-cell helper innate cell population, can be easily missed due to its low frequency and the fact that it only forms upon immunization or infection with glycolipid-containing antigens or microbes. Here, we describe our in-house optimized protocol to detect these mouse NKT(FH) cells by multiparameter flow cytometer.