Ependymal cell differentiation, from monociliated to multiciliated cells.
Methods Cell Biol
; 127: 19-35, 2015.
Article
em En
| MEDLINE
| ID: mdl-25837384
ABSTRACT
Primary and motile cilia differ in their structure, composition, and function. In the brain, primary cilia are immotile signalling organelles present on neural stem cells and neurons. Multiple motile cilia are found on the surface of ependymal cells in all brain ventricles, where they contribute to the flow of cerebrospinal fluid. During development, monociliated ependymal progenitor cells differentiate into multiciliated ependymal cells, thus providing a simple system for studying the transition between these two stages. In this chapter, we provide protocols for immunofluorescence staining of developing ependymal cells in vivo, on whole mounts of lateral ventricle walls, and in vitro, on cultured ependymal cells. We also provide a list of markers we currently use to stain both types of cilia, including proteins at the ciliary membrane and tubulin posttranslational modifications of the axoneme.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cílios
/
Ventrículos Laterais
/
Epêndima
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Células-Tronco Neurais
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Células Ependimogliais
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article