Human Cytochrome P450 21A2, the Major Steroid 21-Hydroxylase: STRUCTURE OF THE ENZYME·PROGESTERONE SUBSTRATE COMPLEX AND RATE-LIMITING C-H BOND CLEAVAGE.

Pallan, Pradeep S; Wang, Chunxue; Lei, Li; Yoshimoto, Francis K; Auchus, Richard J; Waterman, Michael R; Guengerich, F Peter; Egli, Martin

Pallan, Pradeep S; Wang, Chunxue; Lei, Li; Yoshimoto, Francis K; Auchus, Richard J; Waterman, Michael R; Guengerich, F Peter; Egli, Martin.

Afiliação

Pallan PS; From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146 and.
Wang C; From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146 and.
Lei L; From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146 and.
Yoshimoto FK; From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146 and.
Auchus RJ; the Division of Metabolism, Endocrinology, and Diabetes, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109.
Waterman MR; From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146 and.
Guengerich FP; From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146 and f.guengerich@vanderbilt.edu.
Egli M; From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146 and martin.egli@vanderbilt.edu.

J Biol Chem ; 290(21): 13128-43, 2015 May 22.

Article em En | MEDLINE | ID: mdl-25855791

Assuntos

Progesterona/química; Progesterona/metabolismo; Esteroide 21-Hidroxilase/química; Esteroide 21-Hidroxilase/metabolismo; Sequência de Aminoácidos; Animais; Sítios de Ligação; Catálise; Domínio Catalítico; Bovinos; Cristalografia por Raios X; Deutério/química; Humanos; Hidroxilação; Cinética; Dados de Sequência Molecular; Oxirredução; Ligação Proteica; Conformação Proteica; Homologia de Sequência de Aminoácidos; Especificidade por Substrato

Palavras-chave

cytochrome P450; enzyme kinetics; isotope effect; steroidogenesis; x-ray crystallography

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Progesterona / Esteroide 21-Hidroxilase Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article

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