Using peptide arrays created by the SPOT method for defining protein-protein interactions.
Methods Mol Biol
; 1278: 307-20, 2015.
Article
em En
| MEDLINE
| ID: mdl-25859957
ABSTRACT
Evaluating sites of protein-protein interactions can be an arduous task involving extensive mutagenesis work and attempts to express and purify individual proteins in sufficient quantities. Peptide mapping is a useful alternative to traditional methods as it allows rapid detection of regions and/or individual residues important for binding, and it can be readily applied to numerous proteins at once. Here we describe the use of the ResPep SL SPOT method to evaluate protein-protein binding interactions such as that between G-protein ßγ subunits and SNARE proteins, identifying both regions of interest and subsequently individual residues which can then be manipulated in further biochemical assays to confirm their validity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Mapeamento de Interação de Proteínas
/
Subunidades beta da Proteína de Ligação ao GTP
/
Proteínas SNARE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article