C4d deposition is associated with immune cells infiltrating in kidney allograft glomerulitis and peritubular capillaritis.
Ren Fail
; 37(5): 791-7, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25869053
ABSTRACT
OBJECTIVE:
The aim of our study was to determine the amount and composition of immune cells within glomeruli and PTCs and its relationship with C4d deposition. MATERIALS ANDMETHODS:
Immunohistochemistry staining for C4d, CD3, CD68, granzyme B and Foxp3 was used for phenotyping and enumerating immune cells within intracapillaries.RESULTS:
C4d staining was present in 26 biopsy specimens (C4d(+)) and negative in 25 specimens (C4d(-)). The total number of infiltrating cells in glomerulus and PTC in C4d(+) was significantly higher than in C4d(-). Although the C4d(+) showed a significantly higher mean number of macrophages per glomerulus and PTC than in C4d(-) group, the C4d(-) showed a higher mean number of T cells per glomerulus and PTC than in C4d(+). Comparing cell counts in diffuse C4d(+) and focal C4d(+) groups, a significant difference of absolute numbers of intracapillary cells could be observed in glomeruli and PTCs. The mean number of macrophages per glomerulus and PTC in diffuse C4d(+) was greater than that of the focal C4d(+), while mean T cells per glomerulus and PTC were less in cases of diffuse C4d(+) than in focal C4d(+). The differences, however, did not achieve statistical significance. Not only glomerular T cells but also PTCs are granzyme B positive T cells totally.CONCLUSION:
The total number of infiltrating cells in glomeruli and PTC has association with PTC C4d deposition; the infiltrating cells were predominantly macrophages in C4d(+), especially in diffuse C4d(+), whereas the infiltrating cells were predominantly T cells in C4d(-). Glomerular and PTC T cells were cytotoxic phenotype completely.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complicações Pós-Operatórias
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Complemento C4b
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Transplante de Rim
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Aloenxertos
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Glomerulonefrite
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Rejeição de Enxerto
Tipo de estudo:
Risk_factors_studies
Limite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article