Characterization of the recognition specificity of BH2, a monoclonal antibody prepared against the HLA-B27 heavy chain.
Int J Mol Sci
; 16(4): 8142-50, 2015 Apr 13.
Article
em En
| MEDLINE
| ID: mdl-25872138
ABSTRACT
BH2, a monoclonal antibody prepared against the denatured human leukocytic antigen-B27 heavy chain (HLA-B27 HC), can immunoprecipitate the misfolded HLA-B27 HC complexed with Bip in the endoplasmic reticulum and recognize the homodimerized HLA-B27 HC that is often observed on the cell membrane of patients suffered from ankylosing spondylitis (AS). However, the recognition specificity of BH2 toward the other molecules of HLA-B type and toward the different types of HLA molecules remained uncharacterized. In this study, we carried out the HLA-typing by using the Luminex Technology to characterize the recognition specificity of BH2 and analyzed the binding domain of HLA-B27 HC by BH2. Our results indicated that BH2 preferably binds to molecules of HLA-B and -C rather than HLA-A and the binding site is located within the α2 domain of HLA-B27 HC.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sítios de Ligação de Anticorpos
/
Antígeno HLA-B27
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article