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Ex vivo cytosolic delivery of functional macromolecules to immune cells.
Sharei, Armon; Trifonova, Radiana; Jhunjhunwala, Siddharth; Hartoularos, George C; Eyerman, Alexandra T; Lytton-Jean, Abigail; Angin, Mathieu; Sharma, Siddhartha; Poceviciute, Roberta; Mao, Shirley; Heimann, Megan; Liu, Sophia; Talkar, Tanya; Khan, Omar F; Addo, Marylyn; von Andrian, Ulrich H; Anderson, Daniel G; Langer, Robert; Lieberman, Judy; Jensen, Klavs F.
Afiliação
  • Sharei A; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America; The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America; Department of Microbiology and Immunobiology, Harvard Medical School, Boston
  • Trifonova R; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.
  • Jhunjhunwala S; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Hartoularos GC; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Eyerman AT; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Lytton-Jean A; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Angin M; The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America.
  • Sharma S; The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America.
  • Poceviciute R; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Mao S; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Heimann M; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Liu S; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Talkar T; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Khan OF; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Addo M; The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America.
  • von Andrian UH; The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America.
  • Anderson DG; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Langer R; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
  • Lieberman J; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.
  • Jensen KF; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
PLoS One ; 10(4): e0118803, 2015.
Article em En | MEDLINE | ID: mdl-25875117
ABSTRACT
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dextranos / Sistemas de Liberação de Medicamentos / RNA Interferente Pequeno / Dispositivos Lab-On-A-Chip / Anticorpos Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dextranos / Sistemas de Liberação de Medicamentos / RNA Interferente Pequeno / Dispositivos Lab-On-A-Chip / Anticorpos Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article