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Heterogeneous Nuclear Ribonucleoprotein C Proteins Interact with the Human Papillomavirus Type 16 (HPV16) Early 3'-Untranslated Region and Alleviate Suppression of HPV16 Late L1 mRNA Splicing.
Dhanjal, Soniya; Kajitani, Naoko; Glahder, Jacob; Mossberg, Ann-Kristin; Johansson, Cecilia; Schwartz, Stefan.
Afiliação
  • Dhanjal S; From the Department of Laboratory Medicine, Lund University, 221 84 Lund, Sweden.
  • Kajitani N; From the Department of Laboratory Medicine, Lund University, 221 84 Lund, Sweden.
  • Glahder J; From the Department of Laboratory Medicine, Lund University, 221 84 Lund, Sweden.
  • Mossberg AK; From the Department of Laboratory Medicine, Lund University, 221 84 Lund, Sweden.
  • Johansson C; From the Department of Laboratory Medicine, Lund University, 221 84 Lund, Sweden.
  • Schwartz S; From the Department of Laboratory Medicine, Lund University, 221 84 Lund, Sweden Stefan.Schwartz@med.lu.se.
J Biol Chem ; 290(21): 13354-71, 2015 May 22.
Article em En | MEDLINE | ID: mdl-25878250
ABSTRACT
In order to identify cellular factors that regulate human papillomavirus type 16 (HPV16) gene expression, cervical cancer cells permissive for HPV16 late gene expression were identified and characterized. These cells either contained a novel spliced variant of the L1 mRNAs that bypassed the suppressed HPV16 late, 5'-splice site SD3632; produced elevated levels of RNA-binding proteins SRSF1 (ASF/SF2), SRSF9 (SRp30c), and HuR that are known to regulate HPV16 late gene expression; or were shown by a gene expression array analysis to overexpress the RALYL RNA-binding protein of the heterogeneous nuclear ribonucleoprotein C (hnRNP C) family. Overexpression of RALYL or hnRNP C1 induced HPV16 late gene expression from HPV16 subgenomic plasmids and from episomal forms of the full-length HPV16 genome. This induction was dependent on the HPV16 early untranslated region. Binding of hnRNP C1 to the HPV16 early, untranslated region activated HPV16 late 5'-splice site SD3632 and resulted in production of HPV16 L1 mRNAs. Our results suggested that hnRNP C1 controls HPV16 late gene expression.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Regulação Viral da Expressão Gênica / Neoplasias do Colo do Útero / Proteínas Oncogênicas Virais / Splicing de RNA / Regiões 3' não Traduzidas / Ribonucleoproteínas Nucleares Heterogêneas Grupo C / Proteínas do Capsídeo Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Regulação Viral da Expressão Gênica / Neoplasias do Colo do Útero / Proteínas Oncogênicas Virais / Splicing de RNA / Regiões 3' não Traduzidas / Ribonucleoproteínas Nucleares Heterogêneas Grupo C / Proteínas do Capsídeo Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article