Generation and Characterization of Transgenic Mice Expressing Mouse Ins1 Promoter for Pancreatic ß-Cell-Specific Gene Overexpression and Knockout.
Endocrinology
; 156(7): 2724-31, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25885930
ABSTRACT
The technologies for pancreatic ß-cell-specific gene overexpression or knockout are fundamental for investigations of functional genes in vivo. Here we generated the Ins1-Cre-Dsred and Ins1-rtTA mouse models, which expressed the Cre recombinase or reverse tetracycline regulatable transactivator (rtTA) without hGH minigene under the control of mouse Ins1 promoter. Our data showed that the Cre-mediated recombination and rtTA-mediated activation could be efficiently detected at embryonic day 13.5 when these models were crossed with the reporter mice (ROSA(mT/mG) or tetO-HIST1H2BJ/GFP). The Cre and rtTA expression was restricted to ß-cells without leakage in the brain and other tissues. Moreover, both the transgenic lines showed normal glucose tolerance and insulin secretion. These results suggested that the Ins1-Cre-Dsred and Ins1-rtTA mice could be used to knock out or overexpress target genes in embryos and adults to facilitate ß-cell researches.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Camundongos Transgênicos
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Regiões Promotoras Genéticas
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Camundongos Knockout
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Células Secretoras de Insulina
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Técnicas de Introdução de Genes
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Insulina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article