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Functional and mechanistic studies of XPC DNA-repair complex as transcriptional coactivator in embryonic stem cells.
Cattoglio, Claudia; Zhang, Elisa T; Grubisic, Ivan; Chiba, Kunitoshi; Fong, Yick W; Tjian, Robert.
Afiliação
  • Cattoglio C; Department of Molecular and Cell Biology, Howard Hughes Medical Institute, Li Ka Shing Center for Biomedical and Health Sciences, California Institute of Regenerative Medicine Center of Excellence.
  • Zhang ET; Department of Molecular and Cell Biology, Howard Hughes Medical Institute, Li Ka Shing Center for Biomedical and Health Sciences, California Institute of Regenerative Medicine Center of Excellence.
  • Grubisic I; Department of Molecular and Cell Biology, Howard Hughes Medical Institute, Li Ka Shing Center for Biomedical and Health Sciences, California Institute of Regenerative Medicine Center of Excellence, University of California Berkeley-University of California, San Francisco Graduate Program in Bioengin
  • Chiba K; Department of Molecular and Cell Biology, Howard Hughes Medical Institute, Li Ka Shing Center for Biomedical and Health Sciences, California Institute of Regenerative Medicine Center of Excellence.
  • Fong YW; Brigham Regenerative Medicine Center, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Cambridge, MA 02139.
  • Tjian R; Department of Molecular and Cell Biology, Howard Hughes Medical Institute, Li Ka Shing Center for Biomedical and Health Sciences, California Institute of Regenerative Medicine Center of Excellence, jmlim@berkeley.edu.
Proc Natl Acad Sci U S A ; 112(18): E2317-26, 2015 May 05.
Article em En | MEDLINE | ID: mdl-25901318
ABSTRACT
The embryonic stem cell (ESC) state is transcriptionally controlled by OCT4, SOX2, and NANOG with cofactors, chromatin regulators, noncoding RNAs, and other effectors of signaling pathways. Uncovering components of these regulatory circuits and their interplay provides the knowledge base to deploy ESCs and induced pluripotent stem cells. We recently identified the DNA-repair complex xeroderma pigmentosum C (XPC)-RAD23B-CETN2 as a stem cell coactivator (SCC) required for OCT4/SOX2 transcriptional activation. Here we investigate the role of SCC genome-wide in murine ESCs by mapping regions bound by RAD23B and analyzing transcriptional profiles of SCC-depleted ESCs. We establish OCT4 and SOX2 as the primary transcription factors recruiting SCC to regulatory regions of pluripotency genes and identify the XPC subunit as essential for interaction with the two proteins. The present study reveals new mechanistic and functional aspects of SCC transcriptional activity, and thus underscores the diversified functions of this regulatory complex.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica no Desenvolvimento / Proteínas de Ligação a DNA / Células-Tronco Embrionárias Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica no Desenvolvimento / Proteínas de Ligação a DNA / Células-Tronco Embrionárias Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article