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Preliminary Transcriptome Analysis in Lymphoblasts from Cluster Headache and Bipolar Disorder Patients Implicates Dysregulation of Circadian and Serotonergic Genes.
Costa, Marta; Squassina, Alessio; Piras, Ignazio Stefano; Pisanu, Claudia; Congiu, Donatella; Niola, Paola; Angius, Andrea; Chillotti, Caterina; Ardau, Raffaella; Severino, Giovanni; Stochino, Erminia; Deidda, Arianna; Persico, Antonio M; Alda, Martin; Del Zompo, Maria.
Afiliação
  • Costa M; Section of Neuroscience and Clinical Pharmacology, Department of Biomedical Sciences, University of Cagliari, Sp 8 Sestu-Monserrato, Km 0.700, 09042, Monserrato, Cagliari, Italy.
J Mol Neurosci ; 56(3): 688-95, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25912293
Bipolar disorder (BD) and cluster headache (CH) are distinct conditions with important similarities such as a temporal pattern of disturbances, dysregulation of the sleep-wake cycle, and response to lithium treatment in a proportion of patients. Aiming to identify common transcription signatures in these two disorders, we carried out an exploratory microarray gene expression analysis in lymphoblasts from 8 CH and 10 BD I patients selected for positive response to lithium and 10 healthy controls (CO). Gene expression levels of BD and CH were compared with CO to create two lists of differentially expressed genes. We then matched the two lists and focus on genes showing statistically significant difference and same change direction in both disorders. RNA binding motif protein 3 (RBM3) was the most significantly altered gene in the list (3.17 × 10(-13) in BD, 9.44 × 10(-14) in CH). Pathway analysis identified protein processing in endoplasmic reticulum as the most significantly enriched. For validation with quantitative reverse transcription PCR (qRT-PCR) using the same samples, we selected seven genes. Among these, we were able to validate the RBM3, nuclear receptor subfamily 1, group D, member 1 (NR1D1), and tryptophan hydroxylase 1 (TPH1). These genes encode for elements involved in circadian rhythm regulation (RBM3 and NR1D1) and in serotonin synthesis (TPH1), processes previously involved in both disorders, and in the mechanism of action of lithium.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transtorno Bipolar / Serotonina / Cefaleia Histamínica / Relógios Circadianos / Transcriptoma Tipo de estudo: Observational_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transtorno Bipolar / Serotonina / Cefaleia Histamínica / Relógios Circadianos / Transcriptoma Tipo de estudo: Observational_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article