T follicular helper, but not Th1, cell differentiation in the absence of conventional dendritic cells.
J Immunol
; 194(11): 5187-99, 2015 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-25917099
ABSTRACT
Development of long-lived humoral immunity is dependent on CXCR5-expressing T follicular helper (Tfh) cells, which develop concomitantly to effector Th cells that support cellular immunity. Conventional dendritic cells (cDCs) are critical APCs for initial priming of naive CD4(+) T cells but, importantly, also provide accessory signals that govern effector Th cell commitment. To define the accessory role of cDCs during the concurrent development of Tfh and effector Th1 cells, we performed high-dose Ag immunization in conjunction with the Th1-biased adjuvant polyinosinicpolycytidylic acid (pIC). In the absence of cDCs, pIC failed to induce Th1 cell commitment and IgG2c production. However, cDC depletion did not impair Tfh cell differentiation or germinal center formation, and long-lived IgG1 responses of unaltered affinity developed in mice lacking cDCs at the time point for immunization. Thus, cDCs are required for the pIC-driven Th1 cell fate commitment but have no crucial accessory function in relation to Tfh cell differentiation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Poli I-C
/
Linfócitos T Auxiliares-Indutores
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article