Your browser doesn't support javascript.
loading
Meropenem and chromacef intermediates observed in IMP-25 metallo-ß-lactamase-catalyzed hydrolysis.
Oelschlaeger, Peter; Aitha, Mahesh; Yang, Hao; Kang, Joon S; Zhang, Antonia L; Liu, Eleanor M; Buynak, John D; Crowder, Michael W.
Afiliação
  • Oelschlaeger P; Department of Pharmaceutical Sciences, College of Pharmacy, Western University of Health Sciences, Pomona, California, USA poelschlaeger@westernu.edu.
  • Aitha M; Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio, USA.
  • Yang H; Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio, USA.
  • Kang JS; Department of Biological Sciences, California State Polytechnic University, Pomona, California, USA.
  • Zhang AL; Department of Pharmaceutical Sciences, College of Pharmacy, Western University of Health Sciences, Pomona, California, USA.
  • Liu EM; Department of Pharmaceutical Sciences, College of Pharmacy, Western University of Health Sciences, Pomona, California, USA.
  • Buynak JD; Department of Chemistry, Southern Methodist University, Dallas, Texas, USA.
  • Crowder MW; Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio, USA.
Antimicrob Agents Chemother ; 59(7): 4326-30, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25918145
Metallo-ß-lactamases inactivate most ß-lactam antibacterials, and much attention has been paid to their catalytic mechanism. One issue of controversy has been whether ß-lactam hydrolysis generally proceeds through an anionic intermediate bound to the active-site Zn(II) ions or not. The formation of an intermediate has not been shown conclusively in imipenemase (IMP) enzymes to date. Here, we provide evidence that intermediates are formed during the hydrolysis of meropenem and chromacef catalyzed by the variant IMP-25 and, to a lesser degree, IMP-1.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Lactamases / Cefalosporinas / Tienamicinas / Antibacterianos Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Lactamases / Cefalosporinas / Tienamicinas / Antibacterianos Idioma: En Ano de publicação: 2015 Tipo de documento: Article