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Quality evaluation of five commercial enzyme linked immunosorbent assay kits for detecting aflatoxin b1 in feedstuffs.
Sun, Dan-Dan; Gu, Xu; Li, Jun-Guo; Yao, Ting; Dong, Ying-Chao.
Afiliação
  • Sun DD; Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Gu X; Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Li JG; Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Yao T; Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Dong YC; Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Asian-Australas J Anim Sci ; 28(5): 691-6, 2015 May.
Article em En | MEDLINE | ID: mdl-25924961
ABSTRACT
The objective of this study was to evaluate the quality of five commercial enzyme linked immunosorbent assay (ELISA) kits (A, B, C, D, and E) from different suppliers for detecting aflatoxin B1 (AFB1). AFB1-free corn samples supplemented with different levels of AFB1 (5, 10, and 20 µg/kg) were used as positive controls and 6 replicates of each control sample were tested to evaluate the accuracy and precision of these kits. In addition, we also evaluated the performance of these ELISA kits for AFB1 in 30 feed samples, including corn, distillers dried grains with soluble, wheat samples, soybean meal, and poultry feed, which were verified by high performance liquid chromatography. Results showed that the coefficients of variation ranged from 1.18% to 16.22% in intra-plate and 2.85% to 18.04% in inter-plate for the determination of AFB1. The half maximal inhibitory concentration for five kits ranged from 3.72 to 7.22 µg/kg. The quantitation limits of AFB1 were all under the legal limit in China but somewhat inconsistent with kit instructions. Although the recovery rate of four of the five kits were either less than 90% or more than 110%, all these values were acceptable in practice. Two kits had high false positive rates (C and E). In conclusion, our results revealed that the qualities of five tested ELISA kits were significantly different.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2015 Tipo de documento: Article