A Luciferase Reporter Gene Assay to Measure Ebola Virus Viral Protein 35-Associated Inhibition of Double-Stranded RNA-Stimulated, Retinoic Acid-Inducible Gene 1-Mediated Induction of Interferon ß.
J Infect Dis
; 212 Suppl 2: S277-81, 2015 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-25926684
ABSTRACT
During Ebola virus (EBOV) infection, the type I interferon α/ß (IFN-α/ß) innate immune response is suppressed by EBOV viral protein 35 (VP35), a validated drug target. Identification of EBOV VP35 inhibitors requires a cellular system able to assess the VP35-based inhibitory functions of viral double-stranded RNA (dsRNA) IFN-ß induction. We established a miniaturized luciferase gene reporter assay in A549 cells that measures IFN-ß induction by viral dsRNA and is dose-dependently inhibited by VP35 expression. When compared to influenza A virus NS1 protein, EBOV VP35 showed improved inhibition of viral dsRNA-based IFN-ß induction. This assay can be used to screen for EBOV VP35 inhibitors.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
RNA de Cadeia Dupla
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Interferon beta
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Genes Reporter
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Doença pelo Vírus Ebola
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Ebolavirus
/
Luciferases
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article