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Three dimensional time-gated tracking of non-blinking quantum dots in live cells.
DeVore, Matthew S; Stich, Dominik G; Keller, Aaron M; Ghosh, Yagnaseni; Goodwin, Peter M; Phipps, Mary E; Stewart, Michael H; Cleyrat, Cédric; Wilson, Bridget S; Lidke, Diane S; Hollingsworth, Jennifer A; Werner, James H.
Afiliação
  • DeVore MS; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
  • Stich DG; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
  • Keller AM; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
  • Ghosh Y; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
  • Goodwin PM; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
  • Phipps ME; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
  • Stewart MH; Optical Sciences Division, Code 5600, U.S. Naval Research Laboratory, Washington D.C. 20375, USA.
  • Cleyrat C; Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, NM 87131, USA.
  • Wilson BS; Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, NM 87131, USA.
  • Lidke DS; Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, NM 87131, USA.
  • Hollingsworth JA; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
  • Werner JH; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Proc SPIE Int Soc Opt Eng ; 93382015 Mar 12.
Article em En | MEDLINE | ID: mdl-25932286
ABSTRACT
Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. Signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2015 Tipo de documento: Article