[Immortalization of human dental pulp stem cells caused by transferring hTERT gene].
Shanghai Kou Qiang Yi Xue
; 24(2): 135-40, 2015 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-25938139
ABSTRACT
PURPOSE:
To establish an immortalized human dental pulp stem cell line used for basic and clinical research of oral science.METHODS:
Human telomerase reverse transcriptase (hTERT) cDNA was transferred into human dental pulp stem cells (hDPSCs) by lentivirus. The resultant stable clones reproduced successively and the expression of hTERT was identified.RESULTS:
The hTERT gene was transferred into human dental pulp stem cells successfully. The transformed cells expressed telomerase activity and divided vigorously. p35 had been obtained so far.CONCLUSIONS:
The hDPSCs can be immortalized by transferring exogenous hTERT gene to constitute telomerase activity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linhagem Celular Transformada
/
Telomerase
/
Polpa Dentária
/
Células-Tronco Adultas
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2015
Tipo de documento:
Article