Targeted analysis of recombinant NF kappa B (RelA/p65) by denaturing and native top down mass spectrometry.

Savaryn, John Paul; Skinner, Owen S; Fornelli, Luca; Fellers, Ryan T; Compton, Philip D; Terhune, Scott S; Abecassis, Mike M; Kelleher, Neil L

Savaryn, John Paul; Skinner, Owen S; Fornelli, Luca; Fellers, Ryan T; Compton, Philip D; Terhune, Scott S; Abecassis, Mike M; Kelleher, Neil L.

Afiliação

Savaryn JP; Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA; Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Skinner OS; Department of Chemistry, Northwestern University, Evanston, IL, USA.
Fornelli L; Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
Fellers RT; Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
Compton PD; Department of Chemistry, Northwestern University, Evanston, IL, USA; Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
Terhune SS; Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI, USA.
Abecassis MM; Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Kelleher NL; Department of Chemistry, Northwestern University, Evanston, IL, USA; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA; Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA. Electronic address: n-kelleher@northwestern.edu.

J Proteomics ; 134: 76-84, 2016 Feb 16.

Article em En | MEDLINE | ID: mdl-25952688

Assuntos

Espectrometria de Massas; Multimerização Proteica; Fator de Transcrição RelA/química; Fator de Transcrição RelA/metabolismo; Humanos; Isoformas de Proteínas/química; Isoformas de Proteínas/metabolismo; Proteínas Recombinantes/química; Proteínas Recombinantes/metabolismo

Palavras-chave

Bottom up; HaloTag; Mass spectrometry; Native spray; Nuclear factor-kappa b; Phosphorylation; Protein species; Proteoform; RelA; Top down; p65

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Fator de Transcrição RelA / Multimerização Proteica Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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