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Computational analysis of AnmK-like kinase: New insights into the cell wall metabolism of fungi.
Dai, Jianghong; Qu, Hong; Yu, Zhisheng; Yang, Jiangke; Zhang, Hongxun.
Afiliação
  • Dai J; School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, No. 68 Xuefu Road (S), Evergreen Garden, Wuhan 430023, PR China; College of Resources & Environment, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, PR China. Electronic address: daijh
  • Qu H; Center for Bioinformatics, State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, No. 5 Yiheyuan Road, Beijing 100871, PR China. Electronic address: quh@mail.cbi.pku.edu.cn.
  • Yu Z; College of Resources & Environment, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, PR China.
  • Yang J; School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, No. 68 Xuefu Road (S), Evergreen Garden, Wuhan 430023, PR China.
  • Zhang H; College of Resources & Environment, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, PR China.
J Theor Biol ; 379: 59-65, 2015 Aug 21.
Article em En | MEDLINE | ID: mdl-25979372
1,6-Anhydro-N-acetylmuramic acid kinase (AnmK) is the unique enzyme that marks the recycling of the cell wall of Escherichia coli. Here, 81 fungal AnmK-like kinase sequences from 57 fungal species were searched in the NCBI database and a phylogenetic tree was constructed. The three-dimensional structure of an AnmK-like kinase, levoglucosan kinase (LGK) of the yeast Lipomyces starkeyi, was modeled; molecular docking revealed that AnmK and LGK are conserved proteins, and 187Asp, 212Asp are enzymatic residues, respectively. Analysis suggests that 1,6-anhydro-N-acetylglucosamine (anhGlcNAc) and/or 1,6-anhydro-ß-d-glucosamine (anhGlcN) would be the appropriate substrates of AnmK-like kinases. Also, the counterparts of other characteristic enzymes of cell wall recycling of bacteria were found in fungi. Taken together, it is proposed that a putative recycling of anhGlcNAc/anhGlcN, which is associated with the hydrolysis of cell walls, exists in fungi. This computational analysis will provide new insights into the metabolism of fungal cell walls.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfotransferases / Filogenia / Proteínas Fúngicas / Parede Celular / Análise de Sequência de Proteína / Lipomyces Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfotransferases / Filogenia / Proteínas Fúngicas / Parede Celular / Análise de Sequência de Proteína / Lipomyces Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2015 Tipo de documento: Article