Designing Two-Dimensional Protein Arrays through Fusion of Multimers and Interface Mutations.
Nano Lett
; 15(8): 5235-9, 2015 Aug 12.
Article
em En
| MEDLINE
| ID: mdl-25986921
ABSTRACT
We have combined fusion of oligomers with cyclic symmetry and alanine substitutions to eliminate clashes and produce proteins that self-assemble into 2-D arrays upon addition of calcium ions. Using TEM, AFM, small-angle X-ray scattering, and fluorescence microscopy, we show that the designed lattices which are 5 nm high with p3 space group symmetry and 7.25 nm periodicity self-assemble into structures that can exceed 100 µm in characteristic length. The versatile strategy, experimental approach, and hexagonal arrays described herein should prove valuable for the engineering of functional nanostructured materials in 2-D.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salmonella typhimurium
/
Proteínas de Bactérias
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Cálcio
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Substituição de Aminoácidos
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Análise Serial de Proteínas
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Nanoestruturas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article