Two proteins of Cucurbit chlorotic yellows virus, P59 and P9, are self-interacting.
Virus Genes
; 51(1): 152-5, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-26001989
ABSTRACT
The yeast two-hybrid (Y2H) assay, a powerful tool for identifying protein-protein interactions, has been widely used to study viral protein interactions and to elucidate the functions of viral proteins. In this study, Cucurbit chlorotic yellows virus-encoded proteins were investigated by Y2H assays in all possible pairwise combinations, and the self-interactions of P59 and P9 were detected. The interacting domains of P59 and P9 were identified using vectors carrying an activation domain fused to a truncated version of P59 or P9. We found that the middle region (amino acids 173-344) of P59 was necessary for this self-interaction, while three different truncated versions of P9 showed no interaction with full-length P9. This is the first report of the self-interaction of P59 in the genus Crinivirus.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Crinivirus
/
Mapeamento de Interação de Proteínas
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article