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Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.
Chan, Jasper Fuk-Woo; Choi, Garnet Kwan-Yue; Tsang, Alan Ka-Lun; Tee, Kah-Meng; Lam, Ho-Yin; Yip, Cyril Chik-Yan; To, Kelvin Kai-Wang; Cheng, Vincent Chi-Chung; Yeung, Man-Lung; Lau, Susanna Kar-Pui; Woo, Patrick Chiu-Yat; Chan, Kwok-Hung; Tang, Bone Siu-Fai; Yuen, Kwok-Yung.
Afiliação
  • Chan JF; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hon
  • Choi GK; Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
  • Tsang AK; Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
  • Tee KM; Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
  • Lam HY; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.
  • Yip CC; Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
  • To KK; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hon
  • Cheng VC; Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
  • Yeung ML; Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
  • Lau SK; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hon
  • Woo PC; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hon
  • Chan KH; Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
  • Tang BS; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.
  • Yuen KY; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hon
J Clin Microbiol ; 53(8): 2722-6, 2015 Aug.
Article em En | MEDLINE | ID: mdl-26019210
ABSTRACT
Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligonucleotídeos / Sondas de Oligonucleotídeos / Infecções por Coronavirus / Coronavirus / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Adolescent / Adult / Aged / Aged80 / Animals / Child / Child, preschool / Female / Humans / Infant Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligonucleotídeos / Sondas de Oligonucleotídeos / Infecções por Coronavirus / Coronavirus / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Adolescent / Adult / Aged / Aged80 / Animals / Child / Child, preschool / Female / Humans / Infant Idioma: En Ano de publicação: 2015 Tipo de documento: Article