Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.
J Clin Microbiol
; 53(8): 2722-6, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-26019210
ABSTRACT
Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Sondas de Oligonucleotídeos
/
Infecções por Coronavirus
/
Coronavirus
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Animals
/
Child
/
Child, preschool
/
Female
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Humans
/
Infant
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article