[Phylogeny of Neotyphodium endophyte from western Chinese Elymus species based on act sequences].

ABSTRACT

OBJECTIVE: We analyzed phylogenetic relationships between asexual endophytes isolated from western Chinese Elymus species. METHODS: Fifteen act sequences were cloned from asexual endophytes of the western Chinese Elymus species. The phylogenetic tree and network topology structure were constructed using act sequences obtained and Genbank. RESULTS: The act sequences from asexual endophyte were single-copy gene. The western Chinese asexual endophye (Neotyphodium spp.) has a different origination from the North American sexual endophyte ( Epichloë elymi) from Elymus species, but there is a closer relationship between Neotyphodium spp. and Neotyphodium chisosum from North American Achnatherum eminens. Further, endophyte gene-flow exists between sexual Neotyphodium chisosum from North American Achnatherum eminens ( Hap 8) and asexual Neotyphodium sp. from western Chinese Elymus species (Hap 3).