Direct immobilization of antibodies on a new polymer film for fabricating an electrochemical impedance immunosensor.
Anal Biochem
; 485: 81-5, 2015 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-26072006
ABSTRACT
A new polymer bearing aldehyde groups was designed and synthesized by grafting 4-pyridinecarboxaldehyde onto poly(epichlorohydrin). Antibodies can be directly immobilized on the surface of the polymer film through the covalent bonding of aldehyde groups of the film with amino groups of antibodies. In this study, human immunoglobulin G (IgG) was used as a model analyte for the fabrication of an electrochemical impedance immunosensor. Using the proposed immunosensor, IgG in the range from 0.1 to 80 ng ml(-1) was detected with a detection limit of 0.07 ng ml(-1) (signal/noise [S/N]=3). In addition, the electrochemical impedance immunosensor displays good stability and reproducibility.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina G
/
Técnicas Biossensoriais
/
Membranas Artificiais
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article