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Immune system transcriptome in gingival tissues of young nonhuman primates.
Gonzalez, O A; Nagarajan, R; Novak, M J; Orraca, L; Gonzalez-Martinez, J A; Kirakodu, S S; Ebersole, J L.
Afiliação
  • Gonzalez OA; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA.
  • Nagarajan R; Division of Biomedical Informatics, College of Public Health, University of Kentucky, Lexington, KY, USA.
  • Novak MJ; Department of Biostatistics, College of Public Health, University of Kentucky, Lexington, KY, USA.
  • Orraca L; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA.
  • Gonzalez-Martinez JA; School of Dentistry, University of Puerto Rico, San Juan, Puerto Rico.
  • Kirakodu SS; Caribbean Primate Research Center, Sabana Seca, Puerto Rico.
  • Ebersole JL; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA.
J Periodontal Res ; 51(2): 152-63, 2016 Apr.
Article em En | MEDLINE | ID: mdl-26077888
BACKGROUND AND OBJECTIVE: Young/adolescent humans harbor many microorganisms associated with periodontal disease in adults and show substantial gingival inflammatory responses. However, younger individuals do not demonstrate the soft- and hard-tissue destruction that hallmark periodontitis. MATERIAL AND METHODS: This study evaluated responses to the oral microbial ecology in gingival tissues from clinically healthy young Macaca mulatta (< 3 years of age) compared with older animals (5-23 years of age). RNA was isolated from the tissues and analyzed for the transcriptome using the Rhesus Macaque GeneChip (Affymetrix). RESULTS: Global transcriptional profiling of four age groups revealed a subset of 159 genes that were differentially expressed across at least one of the age comparisons. Correlation metrics generated a relevance network abstraction of these genes. Partitioning of the relevance network revealed seven distinct communities comprising functionally related genes associated with host inflammatory and immune responses. A group of genes was identified that were selectively increased/decreased or positively/negatively correlated with gingival profiles in the animals. A principal components analysis created metagenes of expression profiles for classifying the 23 animals. CONCLUSION: The results provide novel system-level insights into gene-expression differences in gingival tissues from healthy young animals, weighted toward host responses associated with anti-inflammatory biomolecules or those linked with T-cell regulation of responses. The combination of the regulated microenvironment may help to explain the apparent 'resistance' of younger individuals to developing periodontal disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Gengiva Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Gengiva Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article