[Molecular authentication of Sailonggu and its resource distribution in Qinghai-Tibet Plateau].
Zhongguo Zhong Yao Za Zhi
; 40(3): 399-403, 2015 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-26084159
ABSTRACT
To provide accurate information on geographic distribution of crude drug Sailonggu in the plateau, we identified zokor species (Eospalax spp.) in Qinghai-Tibet Plateau using molecular methods. Based on the mitochondrial cytochrome B (cytb) gene sequences, we then extracted haplotypes from these sequences and reconstructed phylogenetic trees for the haplotypes using both maximum likelihood (ML) and Bayesian inference (BI) methods. Based on the trees, the species of each sample were determined. Five hundred and three samples from 35 populations were sequenced and their whole cytb sequences (1140 bp) were obtained. From these sequences 150 haplotypes were detected, in which, 126 were Eospalax baileyi, 20 were E. cansus, and 4 were E. smithi of the 35 populations, 28 were E. baileyi type, 5 were E. cansus type, and the remaining 2 were mixed of E. baileyi + E. cansus (DT2) and E. baileyi + E. smithi (ZN3). The results showed that, the regions around the Qinghai lake and near the upper stream of Yellow River started at Guide could be viewed as the producing area of authentic Sailonggu, and also, the cytb gene is a powerful molecular marker to determine the species of zokors as well as for the authentication of geographic distribution of Sailonggu.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Roedores
/
Osso e Ossos
/
Medicina Tradicional Tibetana
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2015
Tipo de documento:
Article