Trichilia catigua ethyl-acetate fraction protects against cognitive impairments and hippocampal cell death induced by bilateral common carotid occlusion in mice.
J Ethnopharmacol
; 172: 232-7, 2015 Aug 22.
Article
em En
| MEDLINE
| ID: mdl-26099636
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Trichilia catigua preparations have antinociceptive, antiinflammatory, and neuroprotective activity. Recently, a neuroprotective role for T. catigua was proposed using an in vitro model of ischemia-reperfusion in rat hippocampal slices. The aim of the present study was to evaluate the effects of an ethyl-acetate fraction (EAF) of T. catigua, which has potent antioxidant activity, in mice subjected to an in vivo model of cerebral ischemia. MATERIAL AND METHODS:
Male Swiss mice were subject to the bilateral common carotid occlusion (BCCAO) model of cerebral ischemia. The animals were orally administered the T. catigua EAF (200, 400, or 800 mg/kg) 30 min before and once per day for 7 days after BCCAO. Histological and behavioral outcomes were assessed using Nissl staining and the Morris water maze test of cognition, respectively.RESULTS:
Mice that were subjected to BCCAO exhibited cognitive impairments in the Morris water maze. The spatial cognitive deficits were counteracted by T. catigua EAF administration (200-800 mg/kg). The T. catigua EAF significantly increased the number of intact-appearing Nissl-stained cells in the hippocampus in BCCAO mice.CONCLUSIONS:
These results show that the T. catigua EAF promoted functional recovery, decreased the delayed hippocampal cell loss, and mitigated the ongoing neurodegenerative processes induced by BCCAO in mice.Palavras-chave
ANOVA=analysis of variance; BCCAO=bilateral common carotid occlusion; Bilateral common carotid occlusion; CE=crude extract; Cognition; DPPH=2,2-diphenyl-1-picryl-hydrazyl; EAF=Trichilia catigua ethyl-acetate fraction; EPC=epicatechin; HPLC=high performance liquid chromatography; Morris water maze; Neurodegeneration; OGD=oxygen and glucose deprivation; PB2=procyanidin B2; ROS=reactive oxygen species; SOD=superoxide dismutase; TFA=trifluoroacetic acid; TGCI=transient global cerebral ischemia; Trichilia catigua ethyl-acetate fraction
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Transtornos Cognitivos
/
Meliaceae
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article