Surface plasmon resonance imaging of pathogens: the Yersinia pestis paradigm.
BMC Res Notes
; 8: 259, 2015 Jun 24.
Article
em En
| MEDLINE
| ID: mdl-26105071
ABSTRACT
BACKGROUND:
Yersinia pestis, causing deadly plague, is classified as a group A bioterrorism bacterium. Some recent DNA-based methods were used for detection of bioterrorism agents.RESULTS:
Y. pestis was used as a model organism to develop an immunosensor based on surface plasmon resonance imaging (SPRi) using monoclonal antibody against Y. pestis F1 antigen. The experimental approach included step-by-step detection of Y. pestis membrane proteins, lysed bacteria, intact bacteria, mock-infected powder and mock-infected clinical specimens. SPRi detected on average 10(6) intact Y. pestis organisms in buffer, in mock-infected powder and in a 14 mixture with HEL cells.CONCLUSIONS:
This study offers the proof-of-concept of the SPRi-based detection of a human pathogen in both environmental and clinical specimens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Yersinia pestis
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article