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Deficiency of sorting nexin 10 prevents bone erosion in collagen-induced mouse arthritis through promoting NFATc1 degradation.
Zhou, Chun; You, Yan; Shen, Weixing; Zhu, Yi-Zhun; Peng, Jing; Feng, Hao-Tian; Wang, Ying; Li, Dong; Shao, Wei-Wei; Li, Cui-Xian; Li, Wan-Zhen; Xu, Jiake; Shen, Xiaoyan.
Afiliação
  • Zhou C; Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
  • You Y; Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
  • Shen W; The Translational Medicine Research Center, Nanjing University of Traditional Chinese Medicine, Nanjing, China.
  • Zhu YZ; Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
  • Peng J; Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
  • Feng HT; School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Western Australia, Australia.
  • Wang Y; Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
  • Li D; Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
  • Shao WW; Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
  • Li CX; Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
  • Li WZ; Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
  • Xu J; School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Western Australia, Australia.
  • Shen X; Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
Ann Rheum Dis ; 75(6): 1211-8, 2016 06.
Article em En | MEDLINE | ID: mdl-26141367
OBJECTIVE: Periarticular and subchondral bone erosion in rheumatoid arthritis caused by osteoclast differentiation and activation is a critical index for diagnosis, therapy and monitoring of the disease. Sorting nexin (SNX) 10, a member of the SNX family which functions in regulation of endosomal sorting, has been implicated to play an important clinical role in malignant osteopetrosis. Here we studied the roles and precise mechanisms of SNX10 in the bone destruction of collagen-induced arthritis (CIA) mice. METHODS: The role of SNX10 in bone destruction was evaluated by a CIA mice model which was induced in male SNX10(-/-) mice and wild type littermates. The mechanism was explored in osteoclasts induced by receptor activator of nuclear factor κB ligand from bone marrow mononuclear cells of wild type and SNX10(-/-) mice. RESULTS: SNX10 knockout prevented bone loss and joint destruction in CIA mice with reduced serum levels of TNF-α, interleukin 1ß and anticollagen IgG 2α antibody. SNX10 deficiency did not block osteoclastogenesis, but significantly impaired osteoclast maturation and bone-resorption function by disturbing the formation of actin belt. The production of TRAP, CtsK and MMP9 in SNX10(-/-) osteoclasts was significantly inhibited, and partially restored by SNX10 overexpression. We further demonstrated that the degradation of NFATc1 was accelerated in SNX10(-/-) osteoclasts causing an inhibition of integrin ß3-Src-PYK2 signalling. CONCLUSIONS: Our study discloses a crucial role and novel mechanism for SNX10 in osteoclast function, and provides evidence for SNX10 as a promising novel therapeutic target for suppression of immune inflammation and bone erosion in rheumatoid arthritis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite Experimental / Reabsorção Óssea / Fatores de Transcrição NFATC / Nexinas de Classificação Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite Experimental / Reabsorção Óssea / Fatores de Transcrição NFATC / Nexinas de Classificação Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article