A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration.
Anal Biochem
; 486: 78-80, 2015 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-26150094
ABSTRACT
Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks the accuracy and reproducibility necessary for use with protein solutions where SDS concentration is a critical factor, so we modified this method after examining multiple parameters (solvent, pH, buffers, and light exposure). The improved method is simple to implement, robust, accurate, and (most important) precise.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dodecilsulfato de Sódio
/
Espectrofotometria
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article