Enzymological description of multitemplate PCR-Shrinking amplification bias by optimizing the polymerase-template ratio.

Ingr, Marek; Dostál, Jirí; Majerová, Tatána

Ingr, Marek; Dostál, Jirí; Majerová, Tatána.

Afiliação

Ingr M; Tomas Bata University in Zlín, Faculty of Technology, Department of Physics and Materials Engineering, Nám. T.G. Masaryka 5555, 76001 Zlín, Czech Republic; Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 128 43 Prague 2, Czech Republic.
Dostál J; Gilead Sciences and IOCB Research Center, Institute of Organic Chemistry and Biochemistry of Academy of Sciences of the Czech Republic, v.v.i., Flemingovo nam. 2, 166 10 Prague 6, Czech Republic.
Majerová T; Gilead Sciences and IOCB Research Center, Institute of Organic Chemistry and Biochemistry of Academy of Sciences of the Czech Republic, v.v.i., Flemingovo nam. 2, 166 10 Prague 6, Czech Republic. Electronic address: tatana.majerova@uochb.cas.cz.

J Theor Biol ; 382: 178-86, 2015 Oct 07.

Article em En | MEDLINE | ID: mdl-26164060

Assuntos

Enzimas/metabolismo; Reação em Cadeia da Polimerase/métodos; Moldes Genéticos; Simulação por Computador; Primers do DNA; Processos Estocásticos

Palavras-chave

Amplicons pooling; Amplification efficiency; Library preparation; Processivity; Species diversity

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Moldes Genéticos / Reação em Cadeia da Polimerase / Enzimas Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google