Isolation of lung multipotent stem cells using a novel microfluidic magnetic activated cell sorting system.
Cell Biol Int
; 39(11): 1348-53, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26174412
ABSTRACT
In recent years, more and more research has shown that the lung is an organ of regenerative potential, with several types of stem/progenitor cells undergoing proliferation and differentiation after lung injury and participating the injury repair process. Mouse lung multipotent stem cells (MLSCs) have extensive self-renewal ability in culture and could differentiate into endothelial and lung epithelial (alveolar epithelial type 1, 2, and Clara) cells in vitro. But the research of MLSCs was limited due to its rarity. In this study, we introduced a novel microfluidic magnetic activated cell sorting system in the isolation of MLSCs. The sorted MLSCs had better viability and purity. They were identified by colony formation efficiency and differentiation ability and they have self-renewal and differentiation capacities, highlighting their stem cell properties.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Separação Imunomagnética
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Células-Tronco Multipotentes
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Técnicas Analíticas Microfluídicas
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Citometria de Fluxo
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Pulmão
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article