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Engineered CHO cells for production of diverse, homogeneous glycoproteins.
Yang, Zhang; Wang, Shengjun; Halim, Adnan; Schulz, Morten Alder; Frodin, Morten; Rahman, Shamim H; Vester-Christensen, Malene B; Behrens, Carsten; Kristensen, Claus; Vakhrushev, Sergey Y; Bennett, Eric Paul; Wandall, Hans H; Clausen, Henrik.
Afiliação
  • Yang Z; 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] The Novo Nordisk Foundation Center for Biosustainability, The Danish Technical University, Denmark.
  • Wang S; 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] The Novo Nordisk Foundation Center for Biosustainability, The Danish Technical University, Denmark.
  • Halim A; 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] The Novo Nordisk Foundation Center for Biosustainability, The Danish Technical University, Denmark.
  • Schulz MA; Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
  • Frodin M; Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark.
  • Rahman SH; 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] Novo Nordisk A/S, Måløv, Denmark.
  • Vester-Christensen MB; 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] Novo Nordisk A/S, Måløv, Denmark.
  • Behrens C; Novo Nordisk A/S, Måløv, Denmark.
  • Kristensen C; Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
  • Vakhrushev SY; Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
  • Bennett EP; Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
  • Wandall HH; Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark.
  • Clausen H; 1] Center for Glycomics, Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark. [2] The Novo Nordisk Foundation Center for Biosustainability, The Danish Technical University, Denmark.
Nat Biotechnol ; 33(8): 842-4, 2015 Aug.
Article em En | MEDLINE | ID: mdl-26192319
ABSTRACT
Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes / Glicoproteínas / Engenharia de Proteínas Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes / Glicoproteínas / Engenharia de Proteínas Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article