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CD4+ROR γ t++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis.
Vonghia, Luisa; Ruyssers, Nathalie; Schrijvers, Dorien; Pelckmans, Paul; Michielsen, Peter; De Clerck, Luc; Ramon, Albert; Jirillo, Emilio; Ebo, Didier; De Winter, Benedicte; Bridts, Chris; Francque, Sven.
Afiliação
  • Vonghia L; Department of Gastroenterology and Hepatology, Antwerp University Hospital, 2560 Antwerp, Belgium ; Department of Basic Medical Sciences, Neuroscience and Sensory Organs, University of Bari, 70100 Bari, Italy.
  • Ruyssers N; Laboratory of Experimental Medicine and Paediatrics, Division of Gastroenterology, University of Antwerp, 2560 Antwerp, Belgium.
  • Schrijvers D; Laboratory of Pharmacology, University of Antwerp, 2560 Antwerp, Belgium.
  • Pelckmans P; Department of Gastroenterology and Hepatology, Antwerp University Hospital, 2560 Antwerp, Belgium ; Laboratory of Experimental Medicine and Paediatrics, Division of Gastroenterology, University of Antwerp, 2560 Antwerp, Belgium.
  • Michielsen P; Department of Gastroenterology and Hepatology, Antwerp University Hospital, 2560 Antwerp, Belgium ; Laboratory of Experimental Medicine and Paediatrics, Division of Gastroenterology, University of Antwerp, 2560 Antwerp, Belgium.
  • De Clerck L; Laboratory of Immunology, Allergology and Rheumatology, University of Antwerp, 2560 Antwerp, Belgium.
  • Ramon A; Department of Gastroenterology and Hepatology, Antwerp University Hospital, 2560 Antwerp, Belgium ; International Tissue Engineering Research Association (ITERA) Life Sciences Forum, 3620 Lanaken, Belgium ; Genetisch-Diagnostisches Labor, 50939 Köln, Germany.
  • Jirillo E; Department of Basic Medical Sciences, Neuroscience and Sensory Organs, University of Bari, 70100 Bari, Italy.
  • Ebo D; Laboratory of Immunology, Allergology and Rheumatology, University of Antwerp, 2560 Antwerp, Belgium.
  • De Winter B; Laboratory of Experimental Medicine and Paediatrics, Division of Gastroenterology, University of Antwerp, 2560 Antwerp, Belgium.
  • Bridts C; Laboratory of Immunology, Allergology and Rheumatology, University of Antwerp, 2560 Antwerp, Belgium.
  • Francque S; Department of Gastroenterology and Hepatology, Antwerp University Hospital, 2560 Antwerp, Belgium ; Laboratory of Experimental Medicine and Paediatrics, Division of Gastroenterology, University of Antwerp, 2560 Antwerp, Belgium.
Mediators Inflamm ; 2015: 239623, 2015.
Article em En | MEDLINE | ID: mdl-26229237
BACKGROUND AND AIMS: Inflammatory mediators that cross-talk in different metabolically active organs are thought to play a crucial role in the pathogenesis of Nonalcoholic Steatohepatitis (NASH). This study was aimed at investigating the CD4+RORγt+ T-helper cells and their counterpart, the CD4+CD25+FOXP3+ regulatory T cells in the liver, subcutaneous adipose tissue (SAT), and abdominal adipose tissue (AAT) in a high fat diet (HFD) mouse model. METHODS: C57BL6 mice were fed a HFD or a normal diet (ND). Liver enzymes, metabolic parameters, and liver histology were assessed. The expression of CD4+RORγt+ cells and regulatory T cells in different organs (blood, liver, AAT, and SAT) were analyzed by flow cytometry. Cytokine and adipokine tissue expression were studied by RT-PCR. RESULTS: Mice fed a HFD developed NASH and metabolic alterations compared to normal diet. CD4+RORγt++ cells were significantly increased in the liver and the AAT while an increase of regulatory T cells was observed in the SAT of mice fed HFD compared to ND. Inflammatory cytokines were also upregulated. CONCLUSIONS: CD4+RORγt++ cells and regulatory T cells are altered in NASH with a site-specific pattern and correlate with the severity of the disease. These site-specific differences are associated with increased cytokine expression.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos CD4 / Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares / Dieta Hiperlipídica / Hepatopatia Gordurosa não Alcoólica Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos CD4 / Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares / Dieta Hiperlipídica / Hepatopatia Gordurosa não Alcoólica Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article