Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents.

Rajendra, Yashas; Balasubramanian, Sowmya; Kiseljak, Divor; Baldi, Lucia; Wurm, Florian M; Hacker, David L

Rajendra, Yashas; Balasubramanian, Sowmya; Kiseljak, Divor; Baldi, Lucia; Wurm, Florian M; Hacker, David L.

Afiliação

Rajendra Y; Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, 1015, Switzerland.
Balasubramanian S; Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, 1015, Switzerland.
Kiseljak D; Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, 1015, Switzerland.
Baldi L; Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, 1015, Switzerland.
Wurm FM; Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, 1015, Switzerland.
Hacker DL; Laboratory for Cellular Biotechnology (LBTC) and Protein Expression Core Facility (PECF), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, 1015, Switzerland.

Biotechnol Prog ; 31(6): 1571-8, 2015.

Article em En | MEDLINE | ID: mdl-26260195

Assuntos

DNA/química; DNA/isolamento & purificação; Plasmídeos/química; Plasmídeos/isolamento & purificação; Transfecção/métodos; Animais; Células CHO; Cricetinae; Cricetulus; Eletroforese em Gel de Poliacrilamida; Interações Hidrofóbicas e Hidrofílicas; Polietilenoimina/química; Solventes/química

Palavras-chave

mammalian cells; plasmid DNA; polar solvent; polyethyleneimine; transfection; transient gene expression

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmídeos / DNA / Transfecção Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article

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