Comparative gene-expression profiling of CD133(+) and CD133(-) D10 melanoma cells.
Future Oncol
; 11(17): 2383-93, 2015.
Article
em En
| MEDLINE
| ID: mdl-26285774
ABSTRACT
AIMS:
The present study aimed to compare the gene-expression profiling of CD133(+) and CD133(-) D10 cells. MATERIALS &METHODS:
Cancer stem cell-like properties and gene-expression profiling of CD133(+) D10 cells versus CD133(-) cells were evaluated.RESULTS:
The CD133(+) D10 cells showed significantly higher clonogenic and spheroid forming potential, also higher expression of stemness genes NANOG and OCT4A compared with the CD133(-) cells. Gene-expression profiling of CD133(+) versus CD133(-) D10 cells revealed that 130 genes including ABC transporter superfamily (ABCC1, ABCG2 and ABCC6) were upregulated, while 61 genes including apoptosis modifying genes (CASP8 and TNFRSF4) were downregulated.CONCLUSION:
We conclude that many genes involved in drug resistance and tumor aggressiveness are upregulated in CD133(+) D10 cells and targeting them might be an efficient strategy for treatment of melanoma.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Glicoproteínas
/
Antígenos CD
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Regulação Neoplásica da Expressão Gênica
/
Perfilação da Expressão Gênica
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Transcriptoma
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Melanoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article