[Elaboration of a national biobank for the study of gestational trophoblastic diseases]. / Création d'une biobanque nationale pour l'étude des maladies trophoblastiques gestationnelles.
J Gynecol Obstet Biol Reprod (Paris)
; 45(6): 559-62, 2016 Jun.
Article
em Fr
| MEDLINE
| ID: mdl-26323857
ABSTRACT
AIM:
To generate a national biobank made up of samples of the highest quality for the purpose of inciting basic research on gestational trophoblastic diseases (GTD). MATERIAL ANDMETHODS:
Three priority axes of research were defined to optimize the nature, method of collection, and storage of the samples. These are to enhance our understanding of GTD, develop new diagnostic tests, and identify new therapeutic targets. The protocol for patient inclusion and sample processing was determined after extensive literature review and collaboration with international experts in the field of GTD.RESULTS:
For each patient with a GTD and for control patients (legally induced abortions), chorionic villi, decidua and tumor samples (fresh, immersed in RNA-protective solution and fixed in formaldehyde), blood (serum, plasma, RNA, and peripheral blood mononuclear cells), urine (supernatant), and cell cultures of villous cytotrophoblasts are prospectively collected. Associations are then made between the collected samples and numerous clinical and biological data, such as human chorionic gonadotropic plasma levels following curettage in the case of a hydatidiform mole.CONCLUSION:
Such a collection of high quality samples and their associated data open up new perspectives for both national and international collaborative research projects.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bancos de Tecidos
/
Doença Trofoblástica Gestacional
Tipo de estudo:
Guideline
Limite:
Adult
/
Female
/
Humans
/
Pregnancy
Idioma:
Fr
Ano de publicação:
2016
Tipo de documento:
Article