Comparison between conventional culture and NAATs for the microbiological diagnosis in gonococcal infection.
Diagn Microbiol Infect Dis
; 83(4): 341-3, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26323884
ABSTRACT
Gonorrhea is a public health problem. Fast diagnostics is necessary. The aim of this study was to compare the diagnostic yield of culture with that of polymerase chain reaction (PCR) in gonococcal infection. This is a study comparing the results of Neisseria gonorrhoeae detection by culture versus PCR from July to December 2012. Molecular diagnosis was performed by real-time PCR using the Versant CT/GC DNA 1.0 assay. In the 768 specimens, 96.9% the results were concordant. In 3.1%, the results were discordant, being PCR-positive and culture-negative in 21 cases and PCR-negative and culture-positive in 3. The sensitivity, specificity, positive predictive value, and negative predictive value for culture were 86.2%, 99.8%, 99.2%, and 96.7%, and for PCR, 98.7%, 100%, 100% and 99.7%, respectively. In laboratories where antimicrobial susceptibility is monitored, an effective approach would be to perform culture in addition to PCR in symptomatic patients.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Gonorreia
/
Técnicas Bacteriológicas
/
Técnicas de Diagnóstico Molecular
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Reação em Cadeia da Polimerase em Tempo Real
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Neisseria gonorrhoeae
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
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Observational_studies
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Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
/
Male
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article