Low-Dose Cadmium Upregulates VEGF Expression in Lung Adenocarcinoma Cells.
Int J Environ Res Public Health
; 12(9): 10508-21, 2015 Aug 28.
Article
em En
| MEDLINE
| ID: mdl-26343694
ABSTRACT
Cadmium (Cd) is a heavy metal and environmental toxin. Exposure to Cd has been associated with a variety of human cancers. In this study, we performed in vitro assays to examine the effects of cadmium chloride (CdCl2) on A549 cells, a human lung adenocarcinoma cell line. Cd does not affect proliferation, migration, or apoptosis of A549 cells at concentrations of 0.1-10 µM. At 0.5 and 1 µM, Cd increases the expression of vascular endothelial growth factor (VEGF) (p < 0.05, p < 0.01, respectively), but not basic fibroblast growth factor (b-FGF) in A549 cells. The conditioned media were collected from the A549 cells treated with 1 µM Cd and were co-cultured with human umbilical vein endothelial cells (HUVECs). Upon treatment with the conditioned media, the proliferation and migration of HUVECs significantly increased (p < 0.01, p < 0.05, respectively), while apoptosis remained unchanged. In addition, 1 µM Cd increases the level of hypoxia inducible factor 1-α (HIF1-α), which is a positive regulator of VEGF expression. Although low-dose Cd does not directly affect the growth of lung adenocarcinoma cells, it might facilitate the development of tumors through its pro-angiogenic effects.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cádmio
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Movimento Celular
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Fator 2 de Crescimento de Fibroblastos
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Apoptose
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Fator A de Crescimento do Endotélio Vascular
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Proliferação de Células
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Poluentes Ambientais
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article