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Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry.
Charretier, Yannick; Dauwalder, Olivier; Franceschi, Christine; Degout-Charmette, Elodie; Zambardi, Gilles; Cecchini, Tiphaine; Bardet, Chloe; Lacoux, Xavier; Dufour, Philippe; Veron, Laurent; Rostaing, Hervé; Lanet, Veronique; Fortin, Tanguy; Beaulieu, Corinne; Perrot, Nadine; Dechaume, Dominique; Pons, Sylvie; Girard, Victoria; Salvador, Arnaud; Durand, Géraldine; Mallard, Frédéric; Theretz, Alain; Broyer, Patrick; Chatellier, Sonia; Gervasi, Gaspard; Van Nuenen, Marc; Roitsch, Carolyn Ann; Van Belkum, Alex; Lemoine, Jérôme; Vandenesch, François; Charrier, Jean-Philippe.
Afiliação
  • Charretier Y; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Dauwalder O; UMR 5280, Institut des Sciences Analytiques, Université de Lyon, Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France.
  • Franceschi C; Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, Bron, France.
  • Degout-Charmette E; CIRI, International Center for Infectiology Research; INSERM U111; CNRS UMR5308; University of Lyon; Ecole Normale Supérieure de Lyon; Lyon, France.
  • Zambardi G; R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
  • Cecchini T; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Bardet C; R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
  • Lacoux X; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Dufour P; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Veron L; R&D ImmunoAssays, Marcy l'Etoile, bioMérieux SA, France.
  • Rostaing H; R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
  • Lanet V; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Fortin T; Technology Research Department, Innovation Unit, Grenoble, bioMérieux SA, France.
  • Beaulieu C; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Perrot N; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Dechaume D; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Pons S; R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
  • Girard V; R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
  • Salvador A; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Durand G; R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
  • Mallard F; UMR 5280, Institut des Sciences Analytiques, Université de Lyon, Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France.
  • Theretz A; R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
  • Broyer P; Technology Research Department, Innovation Unit, Grenoble, bioMérieux SA, France.
  • Chatellier S; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Gervasi G; Technology Research Department, Innovation Unit, Grenoble, bioMérieux SA, France.
  • Van Nuenen M; R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
  • Roitsch CA; Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
  • Van Belkum A; Microbiology Unit, bioMérieux Inc. Durham, NC, USA.
  • Lemoine J; Corporate IP Search and Watch, Intellectual Property, Marcy l'Etoile, bioMérieux SA, France.
  • Vandenesch F; R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
  • Charrier JP; UMR 5280, Institut des Sciences Analytiques, Université de Lyon, Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France.
Sci Rep ; 5: 13944, 2015 Sep 09.
Article em En | MEDLINE | ID: mdl-26350205
ABSTRACT
Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60-80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen Staphylococcus aureus, demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Bactérias / Técnicas de Tipagem Bacteriana Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Bactérias / Técnicas de Tipagem Bacteriana Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article