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Sipa1l3/SPAR3 is targeted to postsynaptic specializations and interacts with the Fezzin ProSAPiP1/Lzts3.
Dolnik, Anna; Kanwal, Noreen; Mackert, Sarah; Halbedl, Sonja; Proepper, Christian; Bockmann, Juergen; Schoen, Michael; Boeckers, Tobias M; Kühl, Susanne J; Schmeisser, Michael J.
Afiliação
  • Dolnik A; Institute for Anatomy and Cell Biology, Ulm University, Ulm, Germany.
  • Kanwal N; Institute for Anatomy and Cell Biology, Ulm University, Ulm, Germany.
  • Mackert S; International Graduate School in Molecular Medicine Ulm, IGradU, Ulm University, Ulm, Germany.
  • Halbedl S; Institute for Anatomy and Cell Biology, Ulm University, Ulm, Germany.
  • Proepper C; International Graduate School in Molecular Medicine Ulm, IGradU, Ulm University, Ulm, Germany.
  • Bockmann J; Institute for Anatomy and Cell Biology, Ulm University, Ulm, Germany.
  • Schoen M; International Graduate School in Molecular Medicine Ulm, IGradU, Ulm University, Ulm, Germany.
  • Boeckers TM; Institute for Anatomy and Cell Biology, Ulm University, Ulm, Germany.
  • Kühl SJ; Institute for Anatomy and Cell Biology, Ulm University, Ulm, Germany.
  • Schmeisser MJ; Institute for Anatomy and Cell Biology, Ulm University, Ulm, Germany.
J Neurochem ; 136(1): 28-35, 2016 Jan.
Article em En | MEDLINE | ID: mdl-26364583
ABSTRACT
Rap GTPase-activating proteins (RapGAPs) are essential for synaptic function as they tightly regulate synaptic Rap signaling. Among the most abundant synaptic RapGAPs in brain are the Spine-associated RapGAPs (SPARs) Sipa1l1/SPAR and Sipa1l2/SPAR2, whereas nothing has been reported on Sipa1l3/SPAR3. In this study, we show that Sipa1l3/SPAR3 is conserved across species, has a distinct expression pattern in the developing rat brain and is localized at excitatory postsynapses. We further demonstrate that the Sipa1l3/SPAR3 C-terminus is required for postsynaptic targeting and represents an interaction module for Fezzins such as ProSAPiP1/Lzts3, a binding partner of the postsynaptic scaffold protein Shank3. Taken together, our data imply that Sipa1l3/SPAR3 is a hitherto unknown synaptic RapGAP, which is targeted to postsynaptic specializations and interacts with Fezzins. Spine-associated RapGAPs (SPARs) are essential modulators of synaptic signaling. Our study is the first to characterize the SPAR family member Sipa1l3/SPAR3 in neuronal tissue. We show that Sipa1l3/SPAR3 is conserved across species, has a distinct expression pattern in brain and is localized to excitatory postsynapses via its C-terminus, which represents an interaction module for other postsynaptic proteins including the Fezzin ProSAPiP1/Lzts3.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinapses / Proteínas de Transporte / Proteínas Ativadoras de GTPase / Proteínas Supressoras de Tumor / Proteínas de Membrana Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinapses / Proteínas de Transporte / Proteínas Ativadoras de GTPase / Proteínas Supressoras de Tumor / Proteínas de Membrana Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2016 Tipo de documento: Article