Development of real-time PCR assays for the detection of Moraxella macacae associated with bloody nose syndrome in rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) macaques.
J Med Primatol
; 44(6): 364-72, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26365904
ABSTRACT
BACKGROUND:
Moraxella macacae is a recently described bacterial pathogen that causes epistaxis or so-called bloody nose syndrome in captive macaques. The aim of this study was to develop specific molecular diagnostic assays for M. macacae and to determine their performance characteristics.METHODS:
We developed six real-time PCR assays on the Roche LightCycler. The accuracy, precision, selectivity, and limit of detection (LOD) were determined for each assay, in addition to further validation by testing nasal swabs from macaques presenting with epistaxis at the Tulane National Primate Research Center.RESULTS:
All assays exhibited 100% specificity and were highly sensitive with an LOD of 10 fg for chromosomal assays and 1 fg for the plasmid assay. Testing of nasal swabs from 10 symptomatic macaques confirmed the presence of M. macacae in these animals.CONCLUSIONS:
We developed several accurate, sensitive, and species-specific real-time PCR assays for the detection of M. macacae in captive macaques.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Moraxellaceae
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Reação em Cadeia da Polimerase em Tempo Real
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Macaca fascicularis
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Macaca mulatta
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Doenças dos Macacos
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Moraxella
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article