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Essential phospholipids prevent islet damage induced by proinflammatory cytokines and hypoxic conditions.
Shahbazov, Rauf; Kanak, Mazhar A; Takita, Morihito; Kunnathodi, Faisal; Khan, Omar; Borenstein, Nofit; Lawrence, Michael C; Levy, Marlon F; Naziruddin, Bashoo.
Afiliação
  • Shahbazov R; Islet Cell Laboratory, Baylor Research Institute, Dallas, TX, USA.
  • Kanak MA; The Institute of Biomedical Studies, Baylor University, Waco, TX, USA.
  • Takita M; Islet Cell Laboratory, Baylor Research Institute, Dallas, TX, USA.
  • Kunnathodi F; Islet Cell Laboratory, Baylor Research Institute, Dallas, TX, USA.
  • Khan O; Islet Cell Laboratory, Baylor Research Institute, Dallas, TX, USA.
  • Borenstein N; Islet Cell Laboratory, Baylor Research Institute, Dallas, TX, USA.
  • Lawrence MC; Islet Cell Laboratory, Baylor Research Institute, Dallas, TX, USA.
  • Levy MF; Baylor Annette C. and Harold C. Simmons Transplant Institute, Baylor University Medical Center, Dallas, TX, USA.
  • Naziruddin B; Baylor Annette C. and Harold C. Simmons Transplant Institute, Baylor University Medical Center, Dallas, TX, USA.
Diabetes Metab Res Rev ; 32(3): 268-77, 2016 Mar.
Article em En | MEDLINE | ID: mdl-26378630
BACKGROUND: The pancreatic islet damage that occurs through an inflammatory response and hypoxia after infusion is a major hurdle in islet transplantation. Because essential phospholipids (EPL) have been shown to exhibit anti-inflammatory properties in liver disease, we analysed their protective effect on islets in inflammatory or hypoxic conditions. METHODS: We evaluated the viability of mouse and human islets cultured with cytokines or in hypoxic conditions for 48 h and measured cytokine expression in islets by quantitative polymerase chain reaction. We then employed an in vivo mouse assay, transplanting a marginal dose of human islets treated with or without EPL into the subcapsule of the kidney in diabetic nude mice and determining the cure rate. RESULTS: The viability of mouse and human islets damaged by cytokines was significantly improved by supplementation of EPL in the culture (p = 0.003 and <0.001 for mouse and human islets respectively). EPL significantly inhibited intracellular expression of IL-1ß and IL-6 in cytokine-damaged human islets (p < 0.001). The viability of human islets in hypoxic conditions was significantly better when treated with EPL (p < 0.001). In the in vivo mouse assay, the EPL-treated islet group had a higher cure rate than the untreated control, with marginal statistical significance (75 and 17% respectively, p = 0.07). CONCLUSIONS: EPL could be a potent agent to protect islets from inflammatory and hypoxic conditions after isolation procedures. Further studies to clarify the effect of EPL in islet transplantation are warranted.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfatidilcolinas / Citocinas / Ilhotas Pancreáticas / Mediadores da Inflamação / Diabetes Mellitus Experimental / Hipóxia Tipo de estudo: Etiology_studies Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfatidilcolinas / Citocinas / Ilhotas Pancreáticas / Mediadores da Inflamação / Diabetes Mellitus Experimental / Hipóxia Tipo de estudo: Etiology_studies Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2016 Tipo de documento: Article