Demonstration of anti-bradykinin compounds in callus cultures of Mandevilla velutina.
Braz J Med Biol Res
; 22(10): 1275-9, 1989.
Article
em En
| MEDLINE
| ID: mdl-2638198
ABSTRACT
Calluses from stems and leaves of Mandevilla velutina were grown in culture for 30, 45 and 60 days. Thin-layer chromatography of ethyl acetate extracts of calluses from stems of M. velutina revealed the presence of 6 compounds. Two of them had RF values identical to those of the anti-bradykinin (BK) compounds MV8609 and MV8610 previously isolated from the natural plant. The ethyl acetate extract (20 to 80 micrograms/ml) from stem callus culture antagonized in a concentration-dependent and reversible manner contractions caused by BK (0.1-1000 nM) in the isolated rat uterus. The extracts obtained from calluses cultured for 30, 45 and 60 days were about 79-, 63- and 31-fold more potent, respectively, in antagonizing BK than the crude extract obtained from the rhizome of the plant.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Bradicinina
/
Extratos Vegetais
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article