Hypolipidemic activity of crude polyphenols from the leaves of Clerodendron colebrookianum Walp in cholesterol fed rats.

ABSTRACT

This study evaluated the hypolipidemic effect of crude polyphenol fraction from Clerodenrdon colebrookianum (CPCC) leaves in cholesterol fed rats. Crude polyphenol fraction was obtained from the ethyl acetate extract of Clerodenrdon colebrookianum (CC). Investigation was conducted by administering graded oral doses (0.25 g, 0.5 g and 1 g/kg b. w. /day) of the CPCC for a period of 28 days. Significant (p < 0.01) rise in plasma total cholesterol (TC), triglycerides (TG), phospholipids (PL), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C) and decrease in high-density lipoprotein cholesterol (HDL-C) were observed in cholesterol fed rats. Increased lipid profile has been depleted and high-density lipoprotein cholesterol (HDL-C) has been increased after chronic feeding of CPCC. In addition, CPCC extract enhanced the excretion of fecal cholesterol (FC) but could not arrest the 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase activities. Histopathological observations showed loss of normal liver architecture in cholesterol fed rats which were retained in CPCC treated groups. Moreover, the analysis of CC extract demonstrated the presence of substantial amount of total polyphenols, flavonoids and tannins content, further HPLC analysis led to the identification and quantification of two most important biologically active secondary metabolites i.e. (+) Catechin (432 ppm) and Quarcetin (105 ppm). The findings of this study suggested that CPCC had a strong hypolipidemic function and could be used as a supplement in healthcare foods and drugs.