Intracellular lumen formation in Drosophila proceeds via a novel subcellular compartment.
Development
; 142(22): 3964-73, 2015 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-26428009
ABSTRACT
Cellular tubes have diverse morphologies, including multicellular, unicellular and subcellular architectures. Subcellular tubes are found prominently within the vertebrate vasculature, the insect breathing system and the nematode excretory apparatus, but how such tubes form is poorly understood. To characterize the cellular mechanisms of subcellular tube formation, we have refined methods of high pressure freezing/freeze substitution to prepare Drosophila larvae for transmission electron microscopic (TEM) analysis. Using our methods, we have found that subcellular tube formation may proceed through a previously undescribed multimembrane intermediate composed of vesicles bound within a novel subcellular compartment. We have also developed correlative light/TEM procedures to identify labeled cells in TEM-fixed larval samples. Using this technique, we have found that Vacuolar ATPase (V-ATPase) and the V-ATPase regulator Rabconnectin-3 are required for subcellular tube formation, probably in a step resolving the intermediate compartment into a mature lumen. In general, our ultrastructural analysis methods could be useful for a wide range of cellular investigations in Drosophila larvae.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compartimento Celular
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ATPases Vacuolares Próton-Translocadoras
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Proteínas de Drosophila
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Espaço Intracelular
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Proteínas Adaptadoras de Transdução de Sinal
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Microscopia Eletrônica de Transmissão
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Drosophila
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article