Gold nanodome-patterned microchips for intracellular surface-enhanced Raman spectroscopy.
Analyst
; 140(24): 8080-7, 2015 Dec 21.
Article
em En
| MEDLINE
| ID: mdl-26438890
ABSTRACT
While top-down substrates for surface-enhanced Raman spectroscopy (SERS) offer outstanding control and reproducibility of the gold nanopatterns and their related localized surface plasmon resonance, intracellular SERS experiments heavily rely on gold nanoparticles. These nanoparticles often result in varying and uncontrollable enhancement factors. Here we demonstrate the use of top-down gold-nanostructured microchips for intracellular sensing. We develop a tunable and reproducible fabrication scheme for these microchips. Furthermore we observe the intracellular uptake of these structures, and find no immediate influence on cell viability. Finally, we perform a proof-of-concept intracellular SERS experiment by the label-free detection of extraneous molecules. By bringing top-down SERS substrates to the intracellular world, we set an important step towards time-dependent and quantitative intracellular SERS.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
/
Análise em Microsséries
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Nanopartículas Metálicas
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Ouro
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article