Crystal structure of the bacteriophage P2 integrase catalytic domain.
FEBS Lett
; 589(23): 3556-63, 2015 Nov 30.
Article
em En
| MEDLINE
| ID: mdl-26453836
ABSTRACT
Bacteriophage P2 is a temperate phage capable of integrating its DNA into the host genome by site-specific recombination upon lysogenization. Integration and excision of the phage genome requires P2 integrase, which performs recognition, cleavage and joining of DNA during these processes. This work presents the high-resolution crystal structure of the catalytic domain of P2 integrase, and analysis of the structure-function relationship of several previously identified non-functional P2 integrase mutants. The DNA binding area is characterized by a large positively charged patch, harboring key residues. The structure reveals potential for large dimer flexibility, likely essential for rearrangement of DNA strands upon integration and excision of the phage DNA.
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01-internacional
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MEDLINE
Assunto principal:
Bacteriófago P2
/
Integrases
/
Domínio Catalítico
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article