Cloning of the short-tailed Gyeongju Donggyeong dog via SCNT: conserving phenotypic inheritance.
J Vet Med Sci
; 78(2): 329-31, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26477541
ABSTRACT
Somatic cell nuclear transfer is a useful tool to maintain genetic information of animals. The Gyeongju Donggyeong dog is a breed registered as natural monument in Korea. The unique feature of the Donggyeong dog is its tail, as the Donggyeong dog can be classified as either short tailed or tailless. The aim of this study was to preserve the Donggyeong dog's unique feature by cloning. Fibroblasts were obtained from a short-tailed Donggyeong dog. In vivo matured oocytes were enucleated, microinjected with a donor cell and fused electrically. Reconstructed embryos were transferred to six recipient dogs. One surrogate became pregnant, and one short-tailed Donggyeong dog was delivered. This study demonstrated that the phenotype of the Donggyeong dog could be conserved by somatic cell nuclear transfer.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clonagem de Organismos
/
Cães
/
Técnicas de Transferência Nuclear
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article