A Photonic Crystal Protein Hydrogel Sensor for Candida albicans.
Angew Chem Int Ed Engl
; 54(44): 13036-40, 2015 Oct 26.
Article
em En
| MEDLINE
| ID: mdl-26480336
ABSTRACT
We report two-dimensional (2D) photonic crystal (PC) sensing materials that selectively detect Candida albicans (C.â
albicans). These sensors utilize Concanavalinâ
A (Conâ
A) protein hydrogels with a 2D PC embedded on the Conâ
A protein hydrogel surface, that multivalently and selectively bind to mannan on the C.â
albicans cell surface to form crosslinks. The resulting crosslinks shrink the Conâ
A protein hydrogel, reduce the 2D PC particle spacing, and blue-shift the light diffracted from the PC. The diffraction shifts can be visually monitored, measured with a spectrometer, or determined from the Debye diffraction ring diameter. Our unoptimized hydrogel sensor has a detection limit of around 32â
CFU/mL for C.â
albicans. This sensor distinguishes between C.â
albicans and those microbes devoid of cell-surface mannan such as the gram-negative bacterium E.â
coli. This sensor provides a proof-of-concept for utilizing recognition between lectins and microbial cell surface carbohydrates to detect microorganisms in aqueous environments.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Candida albicans
/
Técnicas Biossensoriais
/
Concanavalina A
/
Hidrogel de Polietilenoglicol-Dimetacrilato
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article